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rrv e2  (ATCC)


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    ATCC rrv e2
    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Rrv E2, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rrv e2/product/ATCC
    Average 91 stars, based on 21 article reviews
    rrv e2 - by Bioz Stars, 2026-02
    91/100 stars

    Images

    1) Product Images from "A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses"

    Article Title: A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses

    Journal: Vaccines

    doi: 10.3390/vaccines10091374

    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or RRV (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV E2 and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Figure Legend Snippet: TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or RRV (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV E2 and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.

    Techniques Used: RNA Amplification, Transfection, Comparison, Infection, Quantitative RT-PCR, Cotransfection

    Antigen expression from TR-RNAs. ( A ): CHIKV E2 and RRV E2 protein expression in cellular lysates 6 h and 24 h after transfection of 2 μg of replicase-RNA together with 0.5 μg of the indicated TR-RNAs. As control, cells were infected with CHIKV or RRV (MOI 3) or left untreated (ctrl). ( B ): Protein expression 48 h after transfection of 8 μg of the replicase-RNA with 2 μg of the indicated TR-RNAs in cellular lysates or concentrated supernatants. The depicted Western blots are representative of three independent experiments. Uncropped blots and densitometry readings are given in .
    Figure Legend Snippet: Antigen expression from TR-RNAs. ( A ): CHIKV E2 and RRV E2 protein expression in cellular lysates 6 h and 24 h after transfection of 2 μg of replicase-RNA together with 0.5 μg of the indicated TR-RNAs. As control, cells were infected with CHIKV or RRV (MOI 3) or left untreated (ctrl). ( B ): Protein expression 48 h after transfection of 8 μg of the replicase-RNA with 2 μg of the indicated TR-RNAs in cellular lysates or concentrated supernatants. The depicted Western blots are representative of three independent experiments. Uncropped blots and densitometry readings are given in .

    Techniques Used: Expressing, Transfection, Control, Infection, Western Blot



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    rrv e2  (ATCC)
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    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Rrv E2, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti rrv mouse ascites fluid  (ATCC)
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    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Anti Rrv Mouse Ascites Fluid, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rrv mouse ascites fluid  (ATCC)
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    ATCC rrv mouse ascites fluid
    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Rrv Mouse Ascites Fluid, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse rrv immune ascites fluid  (ATCC)
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    ATCC mouse rrv immune ascites fluid
    ( A ) 293T cells were transfected with the <t>RRV</t> structural genes, stained using CHK-265 human IgG1, CHIKV <t>immune</t> <t>IgG,</t> or IgG isolated from healthy controls (healthy control IgG), and analyzed by flow cytometry. An anti-RRV mAb (RRV-130; 10 μg/ml) and human isotype control (WNV hE16; 10 μg/ml) were included as positive and negative controls, respectively. ( B ) Neutralization assay. Purified polyclonal CHIKV-immune IgG was preincubated with 10 2 FFU of RRV or CHIKV-LR then added to Vero cells for 20 or 18 h, respectively. Viral foci were counted and compared to wells without mAb to determine relative infection. Purified IgG from healthy individuals (healthy control IgG) was included as a negative control. ( C - E ) Three-week-old male and female WT mice were administered 300 μg of purified human CHIKV immune IgG or healthy control IgG one day prior to infection with 10 3 FFU RRV. ( C ) Weights were followed for 15 dpi. Graph shows mean ±SEM (n = 5–6 per group; two experiments; student’s t-test of AUC analysis: **** P < 0.0001). ( D ) Serum was collected 1 and 3 dpi and titered by qRT-PCR. ( E ) Viral RNA was quantified from spleen, ipsilateral (i.) and contralateral (c.) quadriceps muscles (quad), and ipsilateral and contralateral ankle at 15 dpi using qRT-PCR. ( D-E ) Bars represent the median (n = 5–6 per group; two experiments; Mann-Whitney test; * P < 0.05, ** P < 0.01). Colored circles indicate CHIKV immune IgG treated mouse with identified RRV variant at 3 dpi: orange circle: E2-K189Q mutation; green circle: E2-D214A mutation. ( F , G ) 293T cells were transfected with the WT, E2-D214A, or E2-K189Q RRV structural genes, stained with ( F ) CHK-265 human IgG1, anti-RRV mAb [RRV-130; 0.04 μg/ml (EC 80 )], human isotype control (WNV hE16), ( G ) CHIKV immune IgG, or healthy control IgG at 30 μg/ml unless otherwise noted, and analyzed by flow cytometry. Relative integrated mean fluorescence intensity (iMFI) was calculated by multiplying the percent positive cells and the median fluorescence intensity for each RRV variant and comparing it to the RRV WT control. Bars indicate mean (four experiments performed in duplicate; One-way ANOVA with a Holm-Sidak’s post-test comparing RRV variants to WT; * P < 0.05, **** P < 0.0001). Ab: antibody.
    Mouse Rrv Immune Ascites Fluid, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or RRV (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV E2 and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.

    Journal: Vaccines

    Article Title: A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses

    doi: 10.3390/vaccines10091374

    Figure Lengend Snippet: TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or RRV (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV E2 and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.

    Article Snippet: Primary antibodies were directed against CHIKV E2 (Eurogentec, Cologne, Germany; custom made), RRV E2 (ATCC VR-1246AF), and β-actin (Sigma, Munich, Germany; no. A5441).

    Techniques: RNA Amplification, Transfection, Comparison, Infection, Quantitative RT-PCR, Cotransfection

    Antigen expression from TR-RNAs. ( A ): CHIKV E2 and RRV E2 protein expression in cellular lysates 6 h and 24 h after transfection of 2 μg of replicase-RNA together with 0.5 μg of the indicated TR-RNAs. As control, cells were infected with CHIKV or RRV (MOI 3) or left untreated (ctrl). ( B ): Protein expression 48 h after transfection of 8 μg of the replicase-RNA with 2 μg of the indicated TR-RNAs in cellular lysates or concentrated supernatants. The depicted Western blots are representative of three independent experiments. Uncropped blots and densitometry readings are given in .

    Journal: Vaccines

    Article Title: A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses

    doi: 10.3390/vaccines10091374

    Figure Lengend Snippet: Antigen expression from TR-RNAs. ( A ): CHIKV E2 and RRV E2 protein expression in cellular lysates 6 h and 24 h after transfection of 2 μg of replicase-RNA together with 0.5 μg of the indicated TR-RNAs. As control, cells were infected with CHIKV or RRV (MOI 3) or left untreated (ctrl). ( B ): Protein expression 48 h after transfection of 8 μg of the replicase-RNA with 2 μg of the indicated TR-RNAs in cellular lysates or concentrated supernatants. The depicted Western blots are representative of three independent experiments. Uncropped blots and densitometry readings are given in .

    Article Snippet: Primary antibodies were directed against CHIKV E2 (Eurogentec, Cologne, Germany; custom made), RRV E2 (ATCC VR-1246AF), and β-actin (Sigma, Munich, Germany; no. A5441).

    Techniques: Expressing, Transfection, Control, Infection, Western Blot

    ( A ) 293T cells were transfected with the RRV structural genes, stained using CHK-265 human IgG1, CHIKV immune IgG, or IgG isolated from healthy controls (healthy control IgG), and analyzed by flow cytometry. An anti-RRV mAb (RRV-130; 10 μg/ml) and human isotype control (WNV hE16; 10 μg/ml) were included as positive and negative controls, respectively. ( B ) Neutralization assay. Purified polyclonal CHIKV-immune IgG was preincubated with 10 2 FFU of RRV or CHIKV-LR then added to Vero cells for 20 or 18 h, respectively. Viral foci were counted and compared to wells without mAb to determine relative infection. Purified IgG from healthy individuals (healthy control IgG) was included as a negative control. ( C - E ) Three-week-old male and female WT mice were administered 300 μg of purified human CHIKV immune IgG or healthy control IgG one day prior to infection with 10 3 FFU RRV. ( C ) Weights were followed for 15 dpi. Graph shows mean ±SEM (n = 5–6 per group; two experiments; student’s t-test of AUC analysis: **** P < 0.0001). ( D ) Serum was collected 1 and 3 dpi and titered by qRT-PCR. ( E ) Viral RNA was quantified from spleen, ipsilateral (i.) and contralateral (c.) quadriceps muscles (quad), and ipsilateral and contralateral ankle at 15 dpi using qRT-PCR. ( D-E ) Bars represent the median (n = 5–6 per group; two experiments; Mann-Whitney test; * P < 0.05, ** P < 0.01). Colored circles indicate CHIKV immune IgG treated mouse with identified RRV variant at 3 dpi: orange circle: E2-K189Q mutation; green circle: E2-D214A mutation. ( F , G ) 293T cells were transfected with the WT, E2-D214A, or E2-K189Q RRV structural genes, stained with ( F ) CHK-265 human IgG1, anti-RRV mAb [RRV-130; 0.04 μg/ml (EC 80 )], human isotype control (WNV hE16), ( G ) CHIKV immune IgG, or healthy control IgG at 30 μg/ml unless otherwise noted, and analyzed by flow cytometry. Relative integrated mean fluorescence intensity (iMFI) was calculated by multiplying the percent positive cells and the median fluorescence intensity for each RRV variant and comparing it to the RRV WT control. Bars indicate mean (four experiments performed in duplicate; One-way ANOVA with a Holm-Sidak’s post-test comparing RRV variants to WT; * P < 0.05, **** P < 0.0001). Ab: antibody.

    Journal: PLoS Pathogens

    Article Title: A cross-reactive antibody protects against Ross River virus musculoskeletal disease despite rapid neutralization escape in mice

    doi: 10.1371/journal.ppat.1008743

    Figure Lengend Snippet: ( A ) 293T cells were transfected with the RRV structural genes, stained using CHK-265 human IgG1, CHIKV immune IgG, or IgG isolated from healthy controls (healthy control IgG), and analyzed by flow cytometry. An anti-RRV mAb (RRV-130; 10 μg/ml) and human isotype control (WNV hE16; 10 μg/ml) were included as positive and negative controls, respectively. ( B ) Neutralization assay. Purified polyclonal CHIKV-immune IgG was preincubated with 10 2 FFU of RRV or CHIKV-LR then added to Vero cells for 20 or 18 h, respectively. Viral foci were counted and compared to wells without mAb to determine relative infection. Purified IgG from healthy individuals (healthy control IgG) was included as a negative control. ( C - E ) Three-week-old male and female WT mice were administered 300 μg of purified human CHIKV immune IgG or healthy control IgG one day prior to infection with 10 3 FFU RRV. ( C ) Weights were followed for 15 dpi. Graph shows mean ±SEM (n = 5–6 per group; two experiments; student’s t-test of AUC analysis: **** P < 0.0001). ( D ) Serum was collected 1 and 3 dpi and titered by qRT-PCR. ( E ) Viral RNA was quantified from spleen, ipsilateral (i.) and contralateral (c.) quadriceps muscles (quad), and ipsilateral and contralateral ankle at 15 dpi using qRT-PCR. ( D-E ) Bars represent the median (n = 5–6 per group; two experiments; Mann-Whitney test; * P < 0.05, ** P < 0.01). Colored circles indicate CHIKV immune IgG treated mouse with identified RRV variant at 3 dpi: orange circle: E2-K189Q mutation; green circle: E2-D214A mutation. ( F , G ) 293T cells were transfected with the WT, E2-D214A, or E2-K189Q RRV structural genes, stained with ( F ) CHK-265 human IgG1, anti-RRV mAb [RRV-130; 0.04 μg/ml (EC 80 )], human isotype control (WNV hE16), ( G ) CHIKV immune IgG, or healthy control IgG at 30 μg/ml unless otherwise noted, and analyzed by flow cytometry. Relative integrated mean fluorescence intensity (iMFI) was calculated by multiplying the percent positive cells and the median fluorescence intensity for each RRV variant and comparing it to the RRV WT control. Bars indicate mean (four experiments performed in duplicate; One-way ANOVA with a Holm-Sidak’s post-test comparing RRV variants to WT; * P < 0.05, **** P < 0.0001). Ab: antibody.

    Article Snippet: Cells were fixed at 20 to 22 hpi and stained with mouse RRV immune ascites fluid (ATCC) followed by an anti-mouse IgG conjugated to HRP.

    Techniques: Transfection, Staining, Isolation, Control, Flow Cytometry, Neutralization, Purification, Infection, Negative Control, Quantitative RT-PCR, Muscles, MANN-WHITNEY, Variant Assay, Mutagenesis, Fluorescence